{"id":24531,"date":"2022-04-20T15:42:10","date_gmt":"2022-04-20T12:42:10","guid":{"rendered":"https:\/\/w3.bilkent.edu.tr\/tr\/?p=24531"},"modified":"2022-04-28T12:05:26","modified_gmt":"2022-04-28T09:05:26","slug":"mbg-semineri-malt1-paracaspace-mediated-crosstalk-of-tgf-%ce%b2-and-nf-%ce%bab-signaling-pathways-serif-senturk-1530-27-nisan-en","status":"publish","type":"post","link":"https:\/\/w3.bilkent.edu.tr\/tr\/mbg-semineri-malt1-paracaspace-mediated-crosstalk-of-tgf-%ce%b2-and-nf-%ce%bab-signaling-pathways-serif-senturk-1530-27-nisan-en\/","title":{"rendered":"MBG Semineri: \u201cMalt1 Paracaspace-mediated Crosstalk of TGF-\u03b2 and NF-\u03baB Signaling Pathways?\u201d, \u015eerif \u015eent\u00fcrk, 15:30 27 Nisan (EN)"},"content":{"rendered":"<div class=\"moz-text-html\" lang=\"x-unicode\">\n<p>\u201cMalt1 Paracaspace-mediated Crosstalk of TGF-\u03b2 and NF-\u03baB Signaling Pathways\u201d<\/p>\n<p>Assoc. Prof. \u015eerif \u015eent\u00fcrk<br \/>\nFunctional Cancer Genomics Group<br \/>\nBiomedicine and Genom Center, Dokuz Eyl\u00fcl University,Izmir<br \/>\nTime: April 27, 2022 03:30 PM<\/p>\n<p>Join Zoom Meeting<br \/>\nPlease contact to the department<br \/>\nHost : I\u015f\u0131k Yulu\u011f<br \/>\nAll interested are cordially invited<\/p>\n<p>Abstract<br \/>\nMucosa-associated lymphoid tissue lymphoma translocation protein 1 (Malt1) is a paracaspase known for its role in the regulation of receptor-stimulated nuclear factor kappa B (NF-\u03baB) pathway activation. Transforming growth factor-\u03b2 (TGF-\u03b2) and NF-\u03baB pathways are two fundamental signaling cascades with key functions in cancer cell proliferation and survival. It is widely recognized that these pathways crosstalk at multiple levels, yet the function of Malt1in this crosstalk has not been addressed. This talk will summarize our recent findings on the interplay between Malt1, and TGF-\u03b2 and NF-\u03baB signaling crosstalk in cancer cells. Experiments were conducted in two TGF-\u03b2 -responsive cell lines, a NSCLC cell line (A549) and an HCC cell line (Huh7). The role of canonical TGF-\u03b2 signaling in Malt1 gene expression was studied by suppressing the pathway using shRNA- and CRISPR\/Cas9-mediated perturbations. The regulation of Malt1 gene expression by TGF-\u03b2 was examined using luciferase reporter assay with Malt1 promoter constructs. TGF-\u03b2 and NF-\u03baB crosstalk was analyzed by immunofluorescence staining of NF-\u03baB subunit p65 (RelA) nuclear translocation as well as p65 reporter assays.<\/p>\n<p>Collectively, we showed that Malt1 expression was upregulated by TGF-\u03b2 in a dose- and timedependent manner, both at the transcript and protein levels. shRNA-mediated silencing of TGFBR1 or Smad3 and Smad4 genes abolished TGF-\u03b2 -induced Malt1 expression, stressing the positive transcriptional regulatory role of canonical TGF-\u03b2 signaling. Furthermore, reporter experiments with Malt1 promoter plasmids as well as CRISPR\/Cas9-based genetic ablation studies and rescue experiments identified the Malt1 gene as a direct target of Smad3. Finally, shRNA knockdown of Malt1 gene inhibited TGF-\u03b2 -induced nuclear translocation of p65 and NF-\u03baB target gene expressions, suggesting that Malt1 is a key mediator of TGF-\u03b2 and NF-\u03baB signaling crosstalk. Further studies may provide mechanistic insights into how Malt1 facilitates the activation of NF-\u03baB signaling by the TGF-\u03b2 pathway.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u201cMalt1 Paracaspace-mediated Crosstalk of TGF-\u03b2 and NF-\u03baB Signaling Pathways\u201d Assoc. Prof. \u015eerif \u015eent\u00fcrk Functional Cancer Genomics Group Biomedicine and Genom Center, Dokuz Eyl\u00fcl University,Izmir Time: April 27, 2022 03:30 PM Join Zoom Meeting Please contact to the department Host : I\u015f\u0131k Yulu\u011f All interested are cordially invited Abstract Mucosa-associated lymphoid [\u2026] <\/p>\n<div class=\"clear\"><\/div>\n<p><a class=\"more_link clearfix\" href=\"https:\/\/w3.bilkent.edu.tr\/tr\/mbg-semineri-malt1-paracaspace-mediated-crosstalk-of-tgf-%ce%b2-and-nf-%ce%bab-signaling-pathways-serif-senturk-1530-27-nisan-en\/\" rel=\"nofollow\">Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[22],"tags":[],"class_list":["post-24531","post","type-post","status-publish","format-standard","hentry","category-eskipostlar"],"_links":{"self":[{"href":"https:\/\/w3.bilkent.edu.tr\/tr\/wp-json\/wp\/v2\/posts\/24531","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/w3.bilkent.edu.tr\/tr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/w3.bilkent.edu.tr\/tr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/w3.bilkent.edu.tr\/tr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/w3.bilkent.edu.tr\/tr\/wp-json\/wp\/v2\/comments?post=24531"}],"version-history":[{"count":1,"href":"https:\/\/w3.bilkent.edu.tr\/tr\/wp-json\/wp\/v2\/posts\/24531\/revisions"}],"predecessor-version":[{"id":24532,"href":"https:\/\/w3.bilkent.edu.tr\/tr\/wp-json\/wp\/v2\/posts\/24531\/revisions\/24532"}],"wp:attachment":[{"href":"https:\/\/w3.bilkent.edu.tr\/tr\/wp-json\/wp\/v2\/media?parent=24531"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/w3.bilkent.edu.tr\/tr\/wp-json\/wp\/v2\/categories?post=24531"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/w3.bilkent.edu.tr\/tr\/wp-json\/wp\/v2\/tags?post=24531"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}